Long-term live cell imaging during differentiation of human iPSC-derived neurons

J Wang; PA Gleeson; L Fourriere

Journal article

Long-term live cell imaging during differentiation of human iPSC-derived neurons

J Wang, PA Gleeson, L Fourriere

STAR Protocols | ELSEVIER | Published : 2023

DOI: 10.1016/j.xpro.2023.102699

Abstract

Live-cell imaging is crucial to appreciate the dynamics and the complexity of cellular interaction processes. However, live-cell imaging of human neurons is challenging due to neuronal sensitivity. Here, we describe a long-term live-cell imaging protocol for neurons derived from human induced pluripotent stem cells. By using an IncuCyte live-cell imaging system, we have obtained information on neuronal dynamics during the different stages of neurogenesis. The protocol has also been developed to monitor the dynamics of the neuronal intracellular organelles. For complete details on the use and execution of this protocol, please refer to Wang et al.1

University of Melbourne Researchers

Paul Gleeson Author

Lou Fourriere-Chea Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by funding from the National Health and Medical Research Council of Australia (APP1163862) . J.W. is supported by a University of Melbourne International Postgraduate Award. The authors acknowledge the facilities and the scientific and technical assistance of the iPSC reprogramming facility-Stem Cell Disease Modelling laboratory, The University of Melbourne, which is supported by Phenomics Australia (PA) . The University of Melbourne provided funds for the purchase of the IncuCyte system. We acknowledge Fiona Houghton for her excellent support in the lab. We acknowledge Alice Pebay, Maciej Daniszewski, and Grace Lingerwood for sharing their stem cells expertise and reagents.